Today the museum is housed in the building of the former German Real Gymnasium. The architecturally significant building was completed in 1913 and served the gymnasium until 1945, when it was used as a secondary industrial school. The latter used the building until 1981. Only since 1982 has the former school belonged to the museum - it was officially opened to the public after extensive reconstruction completed in 1996. The focus of the museum is mainly regional - the main collecting and research area is the territory of the former Most district with overlaps to the surrounding districts.
From the geomorphological point of view, the museum follows three significant landscape units - the Krušné Hory Mountains, the western part of the Bohemian Central Highlands and the Most Basin, whose nature and history are currently covered by three natural science and three social science permanent exhibitions.
The Museum also administered the Litvínov Chateau from 1964 - 2005 and the Memorial to the Victims of World War II in Most from 2000 - 2013 in the former crematorium building in the Most Municipal Cemetery.
